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Image Search Results
Journal: Molecular Medicine Reports
Article Title: Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption
doi: 10.3892/mmr.2016.5358
Figure Lengend Snippet: Modified Pulsatilla decoction treatment significantly reduces the secretion of pro-inflammatory cytokines. (A) mRNA expression levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using reverse transcription-quantitative polymerase chain reaction analysis. (B) Protein levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using ELISA. Data are expressed as the mean ± standard deviation and are representative of three independent experiments. * P<0.05, ** P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; IL, interleukin.
Article Snippet: The protein levels of IL-5 and IL-13 in the supernatants of the colon tissue lysates were measured using Mouse IL-13 Quantikine ELISA kit and
Techniques: Modification, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation, Control, Saline